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ps biosensor lact c2 gfp plasmid  (Addgene inc)


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    Structured Review

    Addgene inc ps biosensor lact c2 gfp plasmid
    Ps Biosensor Lact C2 Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lact+c2+gfp/Lact-C2-GFP+(Plasmid+%2322852)/pmc12731819-38-20-30
    Average 95 stars, based on 59 article reviews
    ps biosensor lact c2 gfp plasmid - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Sequencing:

    Article Title: Early-peaking caspase-7 activity at the plasma membrane drives apoptotic phosphatidylserine exposure
    Article Snippet: For the six variants of pMXs-puro-caspase-FLAG (caspase-7, 3IDR-7, 7IDR-3, 7IDR ED/A -3, 7IDR KR/A -3, and 7IDR D23A -3), the caspase coding sequences were PCR-amplified from pcDNA3.1-caspase-FLAGs and ligated to the Bam HⅠ/ Eco RⅠ-digested pMXs-puro (a gift from T. Kitamura) vector using In-Fusion. .. For pcDNA3.1-HaloTag7-Lact-C2 and pcDNA3.1-HaloTag7-Lact-C2 AAA , the coding sequence of Lact-C2 was PCR-amplified using primers for Lact-C2 or mutation-introducing primers (W26A; TGG to GCC, W33A; TGG to GCC, F34A; TTT to GCC) from Lact-C2-GFP (#22852; Addgene). .. The coding sequence of HaloTag7 was PCR-amplified using a HaloTag-PSMD3 fusion vector (#N2701; Promega).

    Polymerase Chain Reaction:

    Article Title: Early-peaking caspase-7 activity at the plasma membrane drives apoptotic phosphatidylserine exposure
    Article Snippet: For the six variants of pMXs-puro-caspase-FLAG (caspase-7, 3IDR-7, 7IDR-3, 7IDR ED/A -3, 7IDR KR/A -3, and 7IDR D23A -3), the caspase coding sequences were PCR-amplified from pcDNA3.1-caspase-FLAGs and ligated to the Bam HⅠ/ Eco RⅠ-digested pMXs-puro (a gift from T. Kitamura) vector using In-Fusion. .. For pcDNA3.1-HaloTag7-Lact-C2 and pcDNA3.1-HaloTag7-Lact-C2 AAA , the coding sequence of Lact-C2 was PCR-amplified using primers for Lact-C2 or mutation-introducing primers (W26A; TGG to GCC, W33A; TGG to GCC, F34A; TTT to GCC) from Lact-C2-GFP (#22852; Addgene). .. The coding sequence of HaloTag7 was PCR-amplified using a HaloTag-PSMD3 fusion vector (#N2701; Promega).

    Mutagenesis:

    Article Title: Early-peaking caspase-7 activity at the plasma membrane drives apoptotic phosphatidylserine exposure
    Article Snippet: For the six variants of pMXs-puro-caspase-FLAG (caspase-7, 3IDR-7, 7IDR-3, 7IDR ED/A -3, 7IDR KR/A -3, and 7IDR D23A -3), the caspase coding sequences were PCR-amplified from pcDNA3.1-caspase-FLAGs and ligated to the Bam HⅠ/ Eco RⅠ-digested pMXs-puro (a gift from T. Kitamura) vector using In-Fusion. .. For pcDNA3.1-HaloTag7-Lact-C2 and pcDNA3.1-HaloTag7-Lact-C2 AAA , the coding sequence of Lact-C2 was PCR-amplified using primers for Lact-C2 or mutation-introducing primers (W26A; TGG to GCC, W33A; TGG to GCC, F34A; TTT to GCC) from Lact-C2-GFP (#22852; Addgene). .. The coding sequence of HaloTag7 was PCR-amplified using a HaloTag-PSMD3 fusion vector (#N2701; Promega).

    Plasmid Preparation:

    Article Title: Identification of specific lipid-protein interactions in dividing cells using lipid-trap mass spectrometry
    Article Snippet: .. pCW57-GFP-2A-MCS was a gift from Adam Karpf (Addgene plasmid # 71783), Lact-C2-GFP was a gift from Sergio Grinstein (Addgene plasmid # 22852), mCherry-TOMM20-N-10 was a gift from Michael Davidson (Addgene plasmid # 55146). .. RACGAP1 cDNA was a gift from Prof. Francis Barr . pNG72-CHMP2A-LAP-GFP was a gift from Prof. Juan Martin Serrano . pAG138-BFP-Livedrop and pCMS28-GFP-CHMP4B were a gift from Prof. Jeremy Carlton. pcW57 vector was a tetracycline/doxycycline-inducible lentiviral vector, Tet ON. pcW57-RACGAP1-GFP was created by digesting pcW57 with NheI and BamHI and ligating the GFP insert.

    Amplification:

    Article Title: Calcium influx rapidly establishes distinct spatial recruitments of Annexins to cell wounds
    Article Snippet: .. To generate the phosphatidylserine (PS) biosensor, Lact-C2 was amplified from Lact-C2-GFP (Addgene #22852) and fused 5′ to StFP. ..

    Article Title: Calcium influx rapidly establishes distinct spatial recruitments of Annexins to cell wounds.
    Article Snippet: .. 17 To generate the PS biosensor, Lact-C2 was amplified from Lact-C2-GFP (Addgene #22852) 18 and fused 5' to StFP. ..



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